The Biological Facilities Center Chemistry Lab (A231, Bevill Annex) is available to faculty and graduate students for research and has the following instruments:
Beckman LS6500
The Beckman LS 6500 Scintillation System
is a bench top liquid scintillation counter designed to provide highly
accurate, automated counting of the single and dual-labeledsamples. The
half-life and window settings for six isotopes ( 3H, 14C, 125I, 35S, 32P
and 22RN ) are permanently stored in the system.
Lachat QuikChem 8000
The instrument also has an Ion Chromatograph
capability. In this process, ions are separated on a column and detected
using a conductivity detector. The following anions/cations can be processed:
F-, Cl-, NO2-, Br-,
NO3-, HPO4-, SO42-,
Na+, K+, Mg2+, and Ca2+.
The data can be exported to a disc in Excel or as an ASCII file, as well
as printed in a normal format.
The Lachat QuikChem system automates
wet chemical determinations using the principle of flow injection analysis
(FIA). The analytes available are nitrate, nitrite, ammonia, ortho-phosphate,
silica, and chloride. For N and P lowest levels of detection are
1-4 micrograms per liter. Throughput is approximately one hundred
samples per day.
Beckman DU 650 Spectrophotometer
The DU series is a microprocessor controlled spectrophotometer intended for use in quantitative
and qualitative biological research procedures that require spectrophotometric measurements
in the UV-visible region (190 to 1100nm) of the electromagnetic spectrum. The instrument has
three routine measurement modes: fixed wavelength, wavelength scan and kinetics/time. The fixed
wave length mode is used more routinely than the other two. In this mode, data can be collected
in either absorbance or transmittance at up to twelve different wavelengths. The absorbance
readings at each wavelength can be converted to concentration using a user input factor.
Analyses can be automated with the use of sampling devices such as the sipper or batch sampler.
The single cuvette (one cm light path) holder can be replaced by a long (10 cm) light path cuvette holder.
Varian 3400 Gas Chromatograph
There are two 3400 series gas chromatographs available. One is equipped with a flame ionization
detector (FID) and the other with an electron capture detector (ECD). These GC's have an inboard
4 method storage with extensive self-diagnostic capability, dual injector oven and dual ionization
detector oven. The flame detector is autoignite with excellent sensitivity and linearity. The
methods employed will detect methane, acetylene, carbon dioxide and ethylene, using a
fused silica metal column.

Carlo Erba Elemental Analyzer EA 1108 is available for analyses of carbon, hydrogen, nitrogen, sulfur, and oxygen (CHNS-O) in biological and detrital samples. The original analytical method is based on the complete and instantaneous oxidation of the sample by "flash combustion" which converts all organic and inorganic substances into combustion products. The resulting combustion gases pass through a reduction furnace and are swept into the chromatographic column by the carrier gas (helium) where they are separated and detected by a thermal conductivity detector (TCD), which gives an output signal proportional to the concentration of the individual components of the mixture.

Shimadzu
Total Organic Carbon Analyzer TOC-500 is a high-temperature combustion
method used for analysis of dissolved organic carbon (DOC). Ultrapure oxygen
gas is used as both a sparging and carrier gas. Sparged, acidified samples
are injected into quartz combustion tubes containing catalysts prior to
entering the infrared detector to measure CO2. The carbon contents
of environmental samples are compared to a series of standards prepared
with ultrapure water.